Higher Education on Buildings: Case Study in the North Dakota Region

Because of the growing demand for local skilled professionals to improve the health, energy efficiency, and sustainability of residential and commercial buildings in North Dakota, this case study reports the current situation of higher education relating to buildings in the state’s vicinity, including Minnesota, Montana, North Dakota, and South Dakota. In this region, 116 programs relating to buildings were found in 41 postsecondary institutions, and both their majors and courses were then studied with frequency lists. The frequency information was analyzed over nine sets of curriculum areas at both graduate and undergraduate levels for the four states. After the current state of buildings in North Dakota was investigated, strategies were then proposed to rectify current issues regarding higher education on buildings, including but not limited to forming a comprehensive and interdisciplinary program on buildings (e.g., architectural engineering), providing more graduate programs, developing more courses in areas that lack adequate coursework, and increasing student enrollment. These strategies will greatly promote the health, energy efficiency, and sustainability for new and existing buildings in the four-state region of Minnesota, Montana, North Dakota, and South Dakota

X-ray diffraction studies on Mycobacterium smegmatis DNA

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31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

From Problem to Net Positive: The transformation of existing, inefficient building stock

13 pagesThe Net Positive studio is a 7-week studio in the penultimate year of a professional M. Arch. program at the University of Minnesota. This studio focuses on developing, assessing, documenting, and representing interaction between architectural form and environmental factors, using energy modeling tools and incorporating frequent quantitative feedback. In the context of existing-building inefficiency as a major contributor to carbon emissions and climate change, students were assigned an existing building, and were required to demonstrate that passive and active modifications of the building and its envelope could lead to an 80% reduction of its baseline energy use. Within this context, students were asked to (a) selectively modify or replace existing envelope conditions to create a responsive deep-boundary; (b) establish a dialogue between energy-modeling tools and conceptual development; (c) incorporate a Net-Positive contribution beyond energy production; and (d) understand variables that develop concinnity between form, occupancy and function, and environmental factors. These goals were established within a cooperative pedagogy, Shifting Allegiances, organized to promote cooperative collaboration and shared authorship of projects in graduate-level studios. This is achieved by collectivizing the ownership of topical subject areas, to which students variously direct their effort over the course of the studio. The paper discusses the studio’s cooperative structures, the process of establishing an ongoing dialogic between quantitative data and conceptual development, the role of energy modeling within the design process, and the emerging definition of passive and active envelope systems. The paper concludes with a discussion of the studio’s discussion-based, student-led final review

Kleine-Levin Syndrome and Idiopathic Hypersomnia: Spectrum Disorders

Kleine–Levin syndrome (KLS) and idiopathic hypersomnia (IH) are primary sleep disorders of unknown etiologies, which often run a chronic course. The common core symptoms of these syndromes are hypersomnolence and sleep drunkenness, with periodic hypersomnolence and hyperphagia being the prominent symptoms of KLS. Psychiatric manifestations are common to both and include irritability, depression, apathy, inattention and poor concentration. Both disorders are diagnosed clinically and no specific laboratory investigation is available to confirm the diagnosis. We present a case highlighting the overlapping of the symptoms of KLS and IH, producing a complex clinical picture

Sexual Risk Behavior: A Perilous Journey By Unmarried Youth Of District Dehradun, Uttarakhand

Background: Sexual health is an integral part of overall health and well-being and determination of sexuality of the youth is an important milestone in understanding their behavior, associated risks and outcomes. The declining age at puberty and increasing age at marriage has created a longer growing period in which youth may engage in sexual health risk behaviors. This research focuses attention on estimation and involvement of the most dynamic &amp; valuable segment of a nation’s population towards sexual risk behaviors. Aims &amp; objectives: The aim of this study is to find out the prevalence and determinants of sexual health risk behaviors amongst youth in District Dehradun. Material and methods: It was a cross-sectional study conducted over a period of 06 months in rural and urban area of District Dehradun. The study surveyed 1800 male youth aged 15-24 years using a self-administered questionnaire (YRBSS &amp; Big five inventory). After collection, the data was entered using the SPSS software and analyzed using SPSS and Microsoft Excel 2010. Results: Out of 1800 participants, 19% were found to be at risk of sexual health risk behavior and reported having had sex. Out of these 342 at-risk, 43.5% accepted involving in sexual activity even before adulthood, 31% reported having multiple sexual partners and 21.1% accepted not using condoms. Place of residence and personality trait were found to be important determinants of sexual health risk behaviors. Conclusion: Our study documented the high prevalence of sexual risk behavior among male youth of rural and urban area of District Dehradun

Why road safety is the need of the hour? A study among youth of District Dehradun, Uttarakhand

Background: Road traffic injuries are the eighth leading cause of death globally, and the leading cause of death for young people aged 15–29 years. Each year, almost 400,000 young people under 25 years old are killed in a road traffic crash - about 1049 youngsters every day. Aims and objectives: To find out the prevalence of road safety related health risk behaviours and its determinants amongst young males of District Dehradun. Methodology: It was a cross sectional study conducted over 12 months of duration. The study sample comprised of 1800 male youth aged 15-24years studying in various schools and colleges of District Dehradun. A pre-tested and pre-structured questionnaire (YRBSS) was used. The data was entered and analysed using SPSS-version 20.0. Results: Approximately three-fourth of youth reported never using seat-belt while driving and only 4.4% reported always using helmet whereas 24.0% accepted never use of helmet. Approximately one-fourth of the total 1168 at risk subjects in past 30 days, accepted driving a vehicle while drunk and 39.9% reported use of mobile phones while driving. Personality traits (extrovert, neuroticism and lack of direction) turned out to be the major factor in road safety related health risk behaviour. Conclusion: Although, road safety related health risk was found to be more among urban youth as compared to their rural counterparts, yet it was found alarmingly high for both rural and urban study population

X-Ray diffraction studies on Mycobacterium smegmatis DNA

In this report, we present fiber-diffraction studies on Mycobacterium smegmatis DNA with high GC content (70%). This was done as part of the investigation of mycobacteria as the group which includes some of the dreadful pathogens that cause tuberculosis, leprosy, and many other diseases. Recently,it has been shown that the Mycobacterium smegmatis genomic DNA has two distinct stretches, with different GC contents. Such an arrangement of sequences may be of some physical significance for the structure of DNA and, thereby, of physiological significance. Hence, Mycobacterium smegmatis DNA was subjected to x-ray diffraction studies. We observed that at a 1:l Na+:phosphate concentration, this DNA exhibits a characteristic A-pattern (unlike Mycobacterium tuberculosis3) throughout the humidity range 30-98%